CIL:12325, Halteria grandinella, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Halteria grandinella; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:contractile vacuole, contractile vacuole pore, cytoplasm; Biological process:contractile vacuole organization, contractile vacuole discharge, cytoplasm organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12325^* Cite

Description

The contractile vacuole may evacuate through a CV pore located in an indentation of the pellicle not far from the adoral end. TEM taken on 3/18/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 5,000X. Bar = 1µm. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Halteria grandinella
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Carolina Biological Supply Co., NC, U.S.A.
Cellular Component: contractile vacuole; contractile vacuole pore; cytoplasm

Biological Context

Biological Process: contractile vacuole organization; contractile vacuole discharge; cytoplasm organization

Attribution

Names: Richard Allen
Link: http://www5.pbrc.hawaii.edu/allen/

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12325
Archival Resource Key (ARK): ark:/b7295/w9cil12325

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print scanned for Photoshop
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2178px	——
Y	2177px	——