CIL:12316, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Nassula; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:macronucleus, nucleolus, nucleolar part; Biological process:macronucleus organization, nucleolus organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12316^* Cite

Description

Transmission electron micrograph of section through the macronucleus of Nassula sp containing many darkly staining nucleoli.

Technical Details

TEM taken on 4/1/69 by R. Allen with Philips 300 operating at 60kV. Neg. 7,650X. Bar = 1µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:7749) is available in the library for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydration in alcohol and embedding in epoxy resin. Microtome section thickness approximately 75nm. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Nassula
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
Cellular Component: macronucleus; nucleolus; nucleolar part

Biological Context

Biological Process: macronucleus organization; nucleolus organization

Attribution

Names: Richard Allen
Link: http://www5.pbrc.hawaii.edu/allen/

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12316
Archival Resource Key (ARK): ark:/b7295/w9cil12316

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1473px	——
Y	1389px	——