CIL:12312, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Nassula; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:contractile vacuole, contractile vacuole pore, smooth endoplasmic reticulum, cytoplasm; Biological process:contractile vacuole organization, , cytoplasm organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12312^* Cite

Description

Contractile vacuole of Nassula sp. Parts of the single CV appear on two sides of the CV pore. Fields of spongiome abut the filled portions of the CV. TEM taken on 3/27/69 by R. Allen with Philips 300 operating at 60kV. Neg. 3,120X. Bar = 2µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis.Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Nassula
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
Cellular Component: contractile vacuole; contractile vacuole pore; smooth endoplasmic reticulum; cytoplasm

Biological Context

Biological Process: contractile vacuole organization; contractile vacuole pore organization; cytoplasm organization

Attribution

Names: Richard Allen
Link: http://www5.pbrc.hawaii.edu/allen/

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12312
Archival Resource Key (ARK): ark:/b7295/w9cil12312

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2960px	——
Y	3749px	——