CIL:12307, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Nassula; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:oral apparatus, microtubule associated complex, symbiont-containing vacuole; Biological process:oral apparatus organization, microtubule bundle formation, ;

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12307^* Cite

Description

Cytopharyngeal basket in tangential section is composed of a complex of microtubular rods. The lumen of the basket contains many vesicles called cytopharyngeal vesicles. They may provide membrane for the nascent food vacuole, lysosomal and/or lytic enzymes, or all of these. Another role may be to provide vacuolar type ATPases (proton pumps) to the nascent food vacuole. TEM taken on 3/13/69 by R. Allen with Philips 300 operating at 60kV. Neg. 3,570X. Bar = 2µm.

Technical Details

A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm. There is a high resolution version of this image in the library (CIL:39128) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Nassula
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
Cellular Component: oral apparatus; microtubule associated complex; symbiont-containing vacuole

Biological Context

Biological Process: oral apparatus organization; microtubule bundle formation; bacterial symbiont interaction

Attribution

Names: Richard Allen
Link: http://www5.pbrc.hawaii.edu/allen/

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12307
Archival Resource Key (ARK): ark:/b7295/w9cil12307

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1847px	——
Y	2400px	——