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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12306*  Cite 
Description

Endoplasmic reticulum, mitochondria with their tubular cristae and a Golgi stack. Ribosomes are scattered in the cytosol. TEM taken on 3/13/69 by R. Allen with Philips 300 operating at 60kV. Neg. 20,500X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Nassula
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
Cellular Component
cytoplasm
mitochondrion
Golgi apparatus
endoplasmic reticulum
Biological Context
Biological Process
cytoplasm organization
Attribution
Names
Richard Allen
Link
http://www5.pbrc.hawaii.edu/allen/
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12306
Archival Resource Key (ARK)
ark:/b7295/w9cil12306
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop
Data Qualifiers
processed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 2960px ——
Y 3661px ——