CIL:123, Gallus gallus gallus, erythrocyte. CIL. Dataset

NCBI Organism:Gallus gallus gallus; Cell Types:erythrocyte Cell Components:chromosome, telomeric region Biological process:chromatin assembly, chromosome organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:123^* Cite

Description

Telomeres were isolated from chicken erythrocyte nuclei following restriction endonuclease treatment, and imaged by transmission EM after uranyl acetate negative staining. Like its DNA counterpart, telomeric chromatin has have a looped conformation, which likely aids in protection from the rapid degradation that occurs with free DNA ends.

Technical Details

The image was recorded with a FEI Tecnai 12 TEM at 100KV and recorded on a TVIPS 2048x2048 CCD with 2x binning. See Nikitina T, Woodcock CL. 2004. Closed chromatin loops at the ends of chromosomes. J Cell Biol.166:161-5.

Biological Sources

NCBI Organism Classification: Gallus gallus gallus
Cell Type: erythrocyte
Cellular Component: chromosome, telomeric region

Biological Context

Biological Process: chromatin assembly; chromosome organization
Molecular Function: structural constituent of chromatin

Attribution

Names: woodcock, christopher
Published: Nikitina T, Woodcock CL. 2004. Closed chromatin loops at the ends of chromosomes. J Cell Biol.166:161-5.
Pubmed: 2172301

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL123
Archival Resource Key (ARK): ark:/b7295/w9cil123

Imaging

Image Type: charge coupled device (CCD)
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt
Processing History: unprocessed raw image

Sample Preparation

Methods: glutaraldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2048px	0.454nm
Y	2048px	0.454nm