Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of CENP-O(green) at centromeres during mitosis in INCENP OFF cells. Alpha-tubulin is red and DAPI (DNA) is blue See Fig 7C panels 13-16 in Xu et al. 2009 J Cell Biol 187, 637-653.