Cell of the DT40 chicken B lymphocyte line at metaphase. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. In this INCENP-OFF cell, the inner centromere protein INCENP shown in green is barely seen. DAPI stained chromosomes are shown in blue and microtubules in red. See Fig 2A panel 5 in Xu et al. 2009 J Cell Biol 187, 637-653.