CIL:12036, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:axonemal dynein complex, cilium axoneme, axonemal microtubule; Biological process:axonemal dynein complex assembly, cilium axoneme assembly, microtubule-based movement;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12036^* Cite

Description

A segment of the edge of the axoneme showing the outer axonemal dynein arms that extend from the A-subfiber of a doublet to bind to the B subfiber of the adjacent doublet. TEM taken on 4/21/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 31,500X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: axonemal dynein complex; cilium axoneme; axonemal microtubule

Biological Context

Biological Process: axonemal dynein complex assembly; cilium axoneme assembly; microtubule-based movement

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder
Link: http://www5.pbrc.hawaii.edu/allen/

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12036
Archival Resource Key (ARK): ark:/b7295/w9cil12036

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3995px	0.63nm
Y	3684px	0.63nm