CIL:12035, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cilium, cilium axoneme, axonemal dynein complex, trichocyst; Biological process:axonemal dynein complex assembly, ;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12035^* Cite

Description

A high resolution image of portion of an isolated cilium exposing two rows of outer dynein arms arising from subfiber A of each doublet. The heavy chains of the dynein complex are linked to the microtubule by stems that can be seen in this micrograph. Thin protrusions link the dynein heavy chains to the adjacent doublet. Much of this cilium is still covered by the ciliary membrane. Also included in this image is an extruded trichocyst. TEM taken on 6/7/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 19,800X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cilium; cilium axoneme; axonemal dynein complex; trichocyst

Biological Context

Biological Process: axonemal dynein complex assembly; trichocyst discharge

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder
Link: http://www5.pbrc.hawaii.edu/allen/

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12035
Archival Resource Key (ARK): ark:/b7295/w9cil12035

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3263px	1nm
Y	3770px	1nm