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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12025*  Cite 
Description

Movie of meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis that have been experimentally flattened. Time-lapse polarization microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 30/60/90 sec for 2 hr 31 min using a QImaging Retigo EXi CCD camera. Raw images were processed using a 5-frame algorithm (Shribak and Oldenbourg, 2003). The time series used for the movie is included in this grouped set.

Biological Sources
NCBI Organism Classification
Nephrotoma
Nephrotoma suturalis
Cell Type
primary spermatocyte
Cellular Component
spindle
nuclear chromosome
Biological Context
Biological Process
male meiosis chromosome segregation
Attribution
Names
James R. LaFountain
Rudolf Oldenbourg
Published
LaFountain JR, Oldenbourg R (2004) Mol Biol Cell 15, 5346-5355.
Published
Oldenbourg R, Mei G (1995) J Microsc 180, 140-147.
Published
Shribak M, Oldenbourg R (2003) Appl Opt 42, 3009-3017
Pubmed
15385630
Pubmed
8537959
Pubmed
12790452
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12025
Archival Resource Key (ARK)
ark:/b7295/w9cil12025
Grouping This image is part of a group.
Imaging
Image Type
charge coupled device (CCD)
Image Mode
polarization microscopy
Parameters Imaged
UV/visible/IR photons
Source of Contrast
intrinsic birefringence
Visualization Methods
relative retardation (retardance) measurement
Processing History
5-frame algorithm
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 678px 87nm
Y 518px 87nm