CIL:12022, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. CIL. Dataset

NCBI Organism:Nephrotoma, , ; Cell Types:primary spermatocyte Cell Components:spindle, nuclear chromosome; Biological process:male meiosis chromosome segregation

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12022^* Cite

Description

Movie of meiosis I (diakinesis through telophase)in primary spermatocytes of the crane-fly Nephrotoma suturalis that have been experimentally flattened. Flattening prevents completion of cytokinesis. Time-lapse DIC microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 2 min for 180 min using a QImaging Retigo EXi CCD camera. A movie of the time series is based is included in this grouped set.

Biological Sources

NCBI Organism Classification: Nephrotoma; Nephrotoma suturalis
Cell Type: primary spermatocyte
Cellular Component: spindle; nuclear chromosome

Biological Context

Biological Process: male meiosis chromosome segregation

Attribution

Names: James R. LaFountain; Rudolf Oldenbourg
Published: LaFountain JR, et al. (2004) Mol Cell Biol 15, 5724-5732
Pubmed: 15469981

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12022
Archival Resource Key (ARK): ark:/b7295/w9cil12022

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD)
Image Mode: differential interference contrast microscopy
Parameters Imaged: UV/visible/IR photons
Source of Contrast: differences in refractive index
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	678px	87nm
Y	518px	87nm

Time	120 seconds	180