CIL:120, Gallus gallus gallus, erythrocyte. CIL. Dataset

NCBI Organism:Gallus gallus gallus; Cell Types:erythrocyte Cell Components:chromosome, telomeric region Biological process:chromatin assembly

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:120^* Cite

Description

Telomeres isolated from chicken erythrocyte nuclei following restriction endonuclease treatment, and imaged by transmission EM after uranyl acetate negative staining. Image recorded with FEI Tecnai 12 TEM at 100KV and recorded on 2048x2048 CCD with 2x binning. Telomeres have a looped conformation, which likely aids in their protection from the rapid degradation that occurs with free DNA ends.

Biological Sources

NCBI Organism Classification: Gallus gallus gallus
Cell Type: erythrocyte
Cellular Component: chromosome, telomeric region

Biological Context

Biological Process: chromatin assembly
Molecular Function: structural constituent of chromatin

Attribution

Names: woodcock, christopher
Pubmed: 2172301

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL120
Archival Resource Key (ARK): ark:/b7295/w9cil120

Imaging

Image Type: transmission electron microscopy (TEM)
Image Mode: illumination by electrons; detection of electrons; charge coupled device (CCD)
Parameters Imaged: electron density
Source of Contrast: elastic scattering
Visualization Methods: uranyl salt; negative staining
Processing History: unprocessed raw image

Sample Preparation

Methods: glutaraldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2048px	0.545nm
Y	2048px	0.545nm