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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11972*  Cite 
Description

Preparation of the live ciliate Tetrahymena pyriformis stained with a mitochondria-specific probe (Mitotracker green) and viewed with the CSU-10 confocal microscope. The mitochondria become visible as they swim through the confocal plane of focus. Non-confocal background light was superimposed by backlighting through the condenser to make the whole organism visible.

Technical Details

See: S. Inoue and T. Inoue (2002) Direct view high-speed confocal scanner: the CSU-10. Meth Cell Biol 70:87-127.

Biological Sources
NCBI Organism Classification
Tetrahymena pyriformis
Cellular Component
mitochondria
Attribution
Names
Shinya Inoue
Published
S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL11972
Archival Resource Key (ARK)
ark:/b7295/w9cil11972
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
probe for mitochondria
Data Qualifiers
processed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 720px ——
Y 480px ——