CIL:11963, Arbacia punctulata, embryonic cell. CIL. Dataset

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11963^* Cite

Description

The movie illustrates two approaches to understanding the 3D structure of complex objects, using the pluteus stage embryo of the sea urchin Arbacia punctulata. The first sequence is a through-focus DIC series presented as a 'walk through' in the z dimension. The second uses projections at different angles through a z-stack, with the results presented as a 'rocking' series in which the 3D structure becomes more readily apparent.

Technical Details

See: K Okazaki and S Inoue (1976). Crystal properties of the larval sea urchin spicule. Dev Growth Diff 18:413-434; S Inoue and T Inoue (1986) Computer-aided stereomicroscopic video reconstruction and serial display from high-resolution light microscope optical sections. Ann NY Acad Sci 483:392-404.

Biological Sources

NCBI Organism Classification: Arbacia punctulata
Cell Type: embryonic cell

Biological Context

Biological Process

Attribution

Names: Shinya Inoue
Published: S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL11963
Archival Resource Key (ARK): ark:/b7295/w9cil11963

Imaging

Image Type: recorded image
Image Mode: differential interference contrast microscopy
Parameters Imaged: optical path length gradient
Source of Contrast: boundaries between regions with different refractive index
Visualization Methods: visualization of contiguous regions
Data Qualifiers: processed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	720px	——
Y	480px	——