CIL:11960, Chaetopterus sp. 1 Woods Hole MA, oocyte. CIL. Dataset

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11960^* Cite

Description

This is one of 4 grouped time lapse movies (CIL 11958-11962) revealing the responses of a meiotic oocyte spindle to various forms of micromanipulation by a glass microneedle. The needle is inserted, from below, through the egg cortex to the inner pole of the metaphase spindle. This movie, speeded up about 10-fold, illustrates the ability of the spindle to return to its attachment site at the cell cortex after being moved. The spindle is made visible by the use of polarizing optics.

Technical Details

See DA Lutz et al 1988. Micromanipulation of the asymmetric positioning of the maturation spindle in Chaetopterus sp oocytes. Cell Motil Cytoskel 11:83-96. This paper includes stills of similar experiments.

Biological Sources

NCBI Organism Classification: Chaetopterus sp. 1 Woods Hole MA
Cell Type: oocyte
Cellular Component: spindle

Biological Context

Biological Process: meiosis

Attribution

Names: Shinya Inoue
Published: S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL11960
Archival Resource Key (ARK): ark:/b7295/w9cil11960

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: polarization microscopy
Parameters Imaged: retardance
Source of Contrast: form birefringence
Visualization Methods: visualization of contiguous regions
Data Qualifiers: processed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	704px	——
Y	480px	——