CIL:11861, Homo sapiens, epithelial cell. CIL. Dataset

NCBI Organism:Homo sapiens; Cell Types:epithelial cell

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11861^* Cite

Description

Cultured retinal pigment epithelial cell were stably transfected with mCherry-clathrin light chain and EGFP-epsin. Live-cell time lapse images were collected on a Nikon TiE TIRF with a 100x1.49 NA objective (~67nm/pixel) using 120 ms exposures. This time series of mCherry-clathrin light chain is part of an image series that includes a single single dual color image and the time series for the EGFP-epsin as separate files.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: epithelial cell
Cell Line: ARPE-19
Cellular Component: clathrin light chain

Attribution

Names: Allen Liu; Sandra L. Schmid

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL11861
Archival Resource Key (ARK): ark:/b7295/w9cil11861

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: TIRF
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: mCherryFP
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1344px	67nm
Y	1024px	67nm