CIL:10723, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. CIL. Dataset

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10723^* Cite

Description

Movie of anaphase B (spindle elongation) of meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis. Time-lapse polarization microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 30 sec over 70 min using a QImaging Retigo EXi CCD camera. Raw images were processed using 4-frame algorithm (Shribak and Oldenbourg, 2003). The time series of images on which the movie is based is included in this grouped set.

Biological Sources

NCBI Organism Classification: Nephrotoma; Nephrotoma suturalis
Cell Type: primary spermatocyte
Cellular Component: spindle; nuclear chromosome

Biological Context

Biological Process: male meiosis chromosome segregation

Attribution

Names: James R. LaFountain; Rudolf Oldenbourg
Published: LaFountain JR, Oldenbourg R (2004) Mol Biol Cell 15, 5346-5355.
Published: Oldenbourg R, Mei G (1995) J Microsc 180, 140-147
Published: Shriback M, Oldenbourg R (2003) Appl Opt 42, 3009-3017
Pubmed: 15385630
Pubmed: 8537959
Pubmed: 12790452

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10723
Archival Resource Key (ARK): ark:/b7295/w9cil10723

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD); Processed image based on 4 recorded images
Image Mode: polarization microscopy
Parameters Imaged: UV/visible/IR photons
Source of Contrast: intrinsic birefringence
Visualization Methods: relative retardation (retardance) measurement
Processing History: 4-frame algorithm

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	678px	87nm
Y	518px	87nm

Time	30 seconds	140