CIL:10553, Toxoplasma gondii RH. CIL. Dataset

NCBI Organism:Toxoplasma gondii RH; Cell Components:dynein complex, centriole, spindle pole, conoid, cortical microtubule; Biological process:regulation of cell shape, microtubule-based movement, microtubule cytoskeleton organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10553^* Cite

Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged Tgalpha1tubulin and mRFP-tagged TgDLC (dynein light chain). DIC Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X, 1.4 NA. Captured with CoolSnapHQ/ICX285. DIC stack corresponds to raw data stack: CIL# 10551 and deconvolved data stack: CIL# 10552. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii RH
Cell Line: Toxoplasma gondii RH stable cell line
Cellular Component: dynein complex; centriole; spindle pole; conoid; cortical microtubule

Biological Context

Biological Process: regulation of cell shape; microtubule-based movement; microtubule cytoskeleton organization
Molecular Function: ATPase activity, coupled; motor activity; structural constituent of cytoskeleton
Human Disease: toxoplasmosis

Attribution

Names: John Murray (University of Pennsylvania); Ke Hu (Indiana University, Bloomington)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10553
Archival Resource Key (ARK): ark:/b7295/w9cil10553

Grouping This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Image Mode: differential interference contrast microscopy
Parameters Imaged: optical path length gradient
Source of Contrast: boundaries between regions with different refractive index
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	384px	0.071µm
Y	384px	0.071µm
Z	38px	0.2µm