CIL:10552, Toxoplasma gondii RH. CIL. Dataset

NCBI Organism:Toxoplasma gondii RH; Cell Components:dynein complex, centriole, spindle pole, conoid, cortical microtubule; Biological process:regulation of cell shape, microtubule-based movement, microtubule cytoskeleton organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10552^* Cite

Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged Tgalpha1tubulin and mRFP-tagged TgDLC (dynein light chain). Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X, 1.4 NA. Filters: EGFP, ex 450-490 em 500-540, mRFP, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. Deconvolved data stack corresponds to raw data stack:CIL# 10551 and DIC image: CIL# 10553. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii RH
Cell Line: Toxoplasma gondii RH stable cell line
Cellular Component: dynein complex; centriole; spindle pole; conoid; cortical microtubule

Biological Context

Biological Process: regulation of cell shape; microtubule-based movement; microtubule cytoskeleton organization
Molecular Function: ATPase activity, coupled; motor activity; structural constituent of cytoskeleton
Human Disease: toxoplasmosis

Attribution

Names: John Murray (University of Pennsylvania); Ke Hu (Indiana University, Bloomington)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10552
Archival Resource Key (ARK): ark:/b7295/w9cil10552

Grouping This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: Yellow fluorescent proteins from Aequorea; mRFP1
Processing History: 3D-deconvolution
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	384px	0.071µm
Y	384px	0.071µm
Z	38px	0.2µm