CIL:10542, Toxoplasma gondii RH. CIL. Dataset

NCBI Organism:Toxoplasma gondii RH; Cell Components:cortical microtubule, spindle pole, centriole, conoid, inner membrane complex; Biological process:regulation of cell shape, microtubule cytoskeleton organization, cell division;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10542^* Cite

Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images overlaid with DIC. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. Image stack corresponds to raw data stack: CIL# 10545, deconvolved stack: CIL# 10546, and maximum projection stack: CIL# 10547. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii RH
Cell Line: Toxoplasma gondii RH stable cell line
Cellular Component: cortical microtubule; spindle pole; centriole; conoid; inner membrane complex

Biological Context

Biological Process: regulation of cell shape; microtubule cytoskeleton organization; cell division
Molecular Function: structural constituent of cytoskeleton
Human Disease: toxoplasmosis

Attribution

Names: John Murray (University of Pennsylvania); Ke Hu (Indiana University, Bloomington)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10542
Archival Resource Key (ARK): ark:/b7295/w9cil10542

Grouping This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Image Mode: fluorescence microscopy; differential interference contrast microscopy
Parameters Imaged: fluorescence emission; optical path length gradient
Source of Contrast: distribution of a specific protein; boundaries between regions with different refractive index
Visualization Methods: Yellow fluorescent proteins from Aequorea; mCherryFP
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1024px	0.1067µm
Y	1024px	0.1067µm
Z	23px	0.3µm