CIL:10494, Toxoplasma gondii RH. CIL. Dataset

NCBI Organism:Toxoplasma gondii RH; Cell Components:inner membrane complex, centriole, polar ring of apical complex; Biological process:regulation of cell shape, cell division;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10494^* Cite

Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgCentrin2 and mCherry-tagged TgIMC1 (innermembrane complex scaffold protein). Images overlaid with DIC. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 100X oil immersion, 1.35 NA. Filters: EGFP, ex 450-490 em 500-540, mCherry, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. Data stack corresponds to CIL# 10495 and deconvolved data stack CIL 10496. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii RH
Cell Line: Toxoplasma gondii RH stable cell line
Cellular Component: inner membrane complex; centriole; polar ring of apical complex

Biological Context

Biological Process: regulation of cell shape; cell division
Human Disease: toxoplasmosis

Attribution

Names: John Murray (University of Pennsylvania); Ke Hu (Indiana University, Bloomington)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10494
Archival Resource Key (ARK): ark:/b7295/w9cil10494

Grouping This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Image Mode: fluorescence microscopy; differential interference contrast microscopy
Parameters Imaged: fluorescence emission; optical path length gradient
Source of Contrast: distribution of a specific protein; boundaries between regions with different refractive index
Visualization Methods: EGFP; mCherryFP
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	256px	0.0663µm
Y	256px	0.0663µm
Z	7px	0.3µm