CIL:10489, Toxoplasma gondii RH. CIL. Dataset

NCBI Organism:Toxoplasma gondii RH; Cell Components:cortical microtubule Biological process:regulation of cell shape, microtubule cytoskeleton organization, cell division;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10489^* Cite

Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged Tgbeta3-tubulin. 50, 5min timepoints in which daughter cell formation is visible. Images acquired using API DeltaVision on an Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: GFP, ex 450-490 em 500-540. Captured with CoolSnapHQ/ICX285. Deconvolved stack corresponds to raw data stack: CIL# 10488. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii RH
Cell Line: Toxoplasma gondii RH stable cell line
Cellular Component: cortical microtubule

Biological Context

Biological Process: regulation of cell shape; microtubule cytoskeleton organization; cell division
Molecular Function: structural constituent of cytoskeleton
Human Disease: toxoplasmosis

Attribution

Names: John Murray (University of Pennsylvania)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10489
Archival Resource Key (ARK): ark:/b7295/w9cil10489

Grouping This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: 3D-deconvolution
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1024px	0.1067µm
Y	1024px	0.1067µm
Z	8px	0.5µm

Time	304 seconds	50