Toxoplasma gondii expressing EGFP tagged Tgbeta2-tubulin and mCherry-TgDCCP (Double cortin containing protein), an unpublished microtubule binding protein. Images overlaid with DIC. For GFP, ex 450-490 em 500-540; for mCherry ex 555-592 em 600-665. Images acquired using API Delta Vision on a Olympus ix70 wide-field microscope. Objective: Olympus 60X water immersion, 1.2 NA. Captured with CoolSnapHQ/ICX285. 23 images in z-series; this is raw,unprocessed data stack; 3D deconvolved stack is: CIL# 10466. Image is a control not treated with oryzalin. Part of an experiment with some oryzalin-treatment. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
Spatial Axis | Image Size | Pixel Size |
---|---|---|
X | 256px | 0.1067µm |
Y | 256px | 0.1067µm |
Z | 23px | 0.3µm |