CIL:10461, Toxoplasma gondii RH, Toxoplasma gondii RH tachyzoite. CIL. Dataset

NCBI Organism:Toxoplasma gondii RH; Cell Components:cortical microtubule cytoskeleton, microtubule organizing center; Biological process:regulation of cell shape

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10461^* Cite

Description

Toxoplasma gondii expressing EGFP tagged Tgbeta1-tubulin and TgAPR1-mCherry (TgAPR1 is a Microtubule Organizing Center protein). Images overlaid with DIC. For GFP, ex 450-490 em 500-540; for mCherry ex 555-592 em 600-665. Images acquired using API DeltaVision on a Olympus ix70 wide-field microscope. Objective: Olympus 60X water immersion, 1.2 NA. Captured with CoolSnapHQ/ICX285. 15 images in z-series; Image stack corresponds to deconvolved data: CIL# 10458 and raw image stack: CIL# 10457. Unpublished image similar to images published in PMID: 16518471. Also see publication for experimental details.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii RH
Cell Type: Toxoplasma gondii RH tachyzoite
Cell Line: Toxoplasma gondii RH double stable clone expressing TgAPR1-mCherry and EGFP-beta1-tubulin
Cellular Component: cortical microtubule cytoskeleton; microtubule organizing center

Biological Context

Biological Process: regulation of cell shape
Molecular Function: structural constituent of cytoskeleton
Human Disease: toxoplasmosis

Attribution

Names: John Murray

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10461
Archival Resource Key (ARK): ark:/b7295/w9cil10461

Grouping This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Image Mode: fluorescence microscopy; differential interference contrast microscopy
Parameters Imaged: fluorescence emission; optical path length gradient
Source of Contrast: distribution of a specific protein; boundaries between regions with different refractive index
Visualization Methods: EGFP; mCherryFP
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	106.7nm
Y	512px	106.7nm
Z	15px	300nm