CIL:10460, Toxoplasma gondii RH, Toxoplasma gondii RH. CIL. Dataset

NCBI Organism:Toxoplasma gondii RH; Cell Components:cortical microtubule, transverse to long axis, microtubule organizing center; Biological process:regulation of cell shape

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10460^* Cite

Description

Toxoplasma gondii double stable clone expressing TgAPR1-mCherryFP (an MTOC protein) and EGFP-beta1-tubulin. 3D stack image of a live unfixed specimen acquired using API Delta Vision on Olympus ix70 wide-field microscope with Cool SNAP HQ/ICX285 CCD camera. Filter set: EGFP excitation 450-490 and emission 500-540; mCherry excitation 555-592 and emission: 600-665. Dichromatic mirror: Chroma 89021. Objective: Olympus 60x water immersion, 1.2 NA. This raw data stack with DIC corresponds to raw data stack: CIL# 10454 and deconvolved stack: CIL# 10455. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii RH
Cell Type: Toxoplasma gondii RH
Cell Line: Toxoplasma gondii RH double stable clone expressing TgAPR1-mCherryFP and EGFP-beta1-tubulin
Cellular Component: cortical microtubule, transverse to long axis; microtubule organizing center

Biological Context

Biological Process: regulation of cell shape
Molecular Function: structural constituent of cytoskeleton
Human Disease: toxoplasmosis

Attribution

Names: John Murray

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10460
Archival Resource Key (ARK): ark:/b7295/w9cil10460

Grouping This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Image Mode: fluorescence microscopy; boundaries between regions with different refractive index
Parameters Imaged: fluorescence emission; optical path length gradient
Source of Contrast: distribution of a specific protein; boundaries between regions with different refractive index
Visualization Methods: EGFP; mCherryFP
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	384px	106.7nm
Y	384px	106.7nm
Z	15px	300nm