Toxoplasma gondii double stable clone expressing TgAPR1-mCherryFP (an MTOC protein) and EGFP-beta1-tubulin. 3D stack image of a live unfixed specimen acquired using API Delta Vision on Olympus ix70 wide-field microscope with Cool SNAP HQ/ICX285 CCD camera. Filter set: EGFP excitation 450-490 and emission 500-540; mCherry excitation 555-592 and emission: 600-665. Dichromatic mirror: Chroma 89021. Objective: Olympus 60x water immersion, 1.2 NA. Raw data corresponds to raw data stack with DIC: CIL# 10460 and deconvolved stack: CIL# 10455. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.