CIL:10249, Halteria grandinella, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Halteria grandinella; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:macronucleus, nucleolus; Biological process:DNA replication checkpoint, nucleus organization;

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10249^* Cite

Description

The DNA replication band in macronucleus of Halteria. Like hypotrichs the macronucleus of this oligotrich has a DNA replication band where DNA is synthesized. The nucleolus is evident. TEM taken on 3/9/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 13,750X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:7601) is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Halteria grandinella
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Carolina Biological Supply Co., NC, U.S.A.
Cellular Component: macronucleus; nucleolus

Biological Context

Biological Process: DNA replication checkpoint; nucleus organization
Molecular Function: focal point of DNA synthesis

Attribution

Names: Richard Allen
Link: http://www5.pbrc.hawaii.edu/allen/

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10249
Archival Resource Key (ARK): ark:/b7295/w9cil10249

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2207px	——
Y	2375px	——