CIL:10136, Homo sapiens, epithelial cell, human retinal pigmented epithelial cell. CIL. Dataset

NCBI Organism:Homo sapiens; Cell Types:epithelial cell, ; Cell Components:clathrin coat of coated pit, ;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10136^* Cite

Description

Visualization of clathrin coated pit dynamics. Total internal reflection fluorescencec microscopy (TIRF-FM) was performed on live human retinal pigment epithelial (RPE) cells stably expressing 'clathrin light chain a' tagged with EGFP (EGFP-LCa) using a 100x 1.45 NA objective (Nikon) mounted on a Nikon TE2000U inverted microscope (Nikon) coupled to a 14-bit mode operated Hamamatsu Orca II-ERG camera. This single channel TIR-FM movie, showing clathrin coated pit dynamics at the ventral plasma membrane, was acquired at a frame rate of 1s with a 70 msec exposure time during 10 min. Pixel size: 67 nm

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: epithelial cell; human retinal pigmented epithelial cell
Cellular Component: clathrin coat of coated pit; clathrin light chain a

Biological Context

Biological Process

Attribution

Names: Marcel Mettlen; Sandra L. Schmid
OTHER: The Scripps Research Institute

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10136
Archival Resource Key (ARK): ark:/b7295/w9cil10136

Imaging

Image Type: recorded image
Image Mode: TIRF
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	613px	67µm
Y	991px	67µm