CIL:10018, Homo sapiens, permanent cell line cell, epithelial cell. CIL. Dataset

NCBI Organism:Homo sapiens; Cell Types:permanent cell line cell, epithelial cell; Cell Components:Golgi apparatus, endoplasmic reticulum; Biological process:Golgi organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10018^* Cite

Description

Organization of Golgi and endoplasmic reticulum (ER) in a Human HeLa cell expressing Sialyl Transferase-EGFP (green) and Invariant Chain33-mRFP (red). The Sialyl Transferase-EGFP is a Golgi marker and the Invariant Chain33-mRFP is retained in the ER. Zeiss Spinning Disc confocal used with with 220 ms exposure and a 63X oil objective.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: permanent cell line cell; epithelial cell
Cell Line: HeLa
Cellular Component: Golgi apparatus; endoplasmic reticulum

Biological Context

Biological Process: Golgi organization
Molecular Function: endoplasmic reticulum organization

Attribution

Names: Prabuddha Sengupta

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10018
Archival Resource Key (ARK): ark:/b7295/w9cil10018

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: spinning disk confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: mRFP1; EGFP
Processing History: channels merged as color

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	0.11µm
Y	512px	0.11µm
Z	——	0.27µm