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NCBI Organism:Homo sapiens; Cell Types:permanent cell line cell, epithelial cell; Cell Components:Golgi apparatus, endoplasmic reticulum; Biological process:Golgi organization
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10017*  Cite 
Description

Organization of Golgi and endoplasmic reticulum (ER) in a Human HeLa cell expressing Sialyl Transferase-EGFP (green) and Invariant Chain33-mRFP (red). The Sialyl Transferase-EGFP is a Golgi marker and the Invariant Chain33-mRFP is retained in the ER. There are two related files for this specimen: the z series and the maximum projection. This file is the maximum projection. Zeiss Spinning Disc confocal used with with 220 ms exposure and a 63X oil objective.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
permanent cell line cell
epithelial cell
Cell Line
HeLa
Cellular Component
Golgi apparatus
endoplasmic reticulum
Biological Context
Biological Process
Golgi organization
Molecular Function
endoplasmic reticulum organization
Attribution
Names
Prabuddha Sengupta
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10017
Archival Resource Key (ARK)
ark:/b7295/w9cil10017
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
mRFP1
Processing History
maximum projection
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 0.11µm
Y 512px 0.11µm