CIL:10016, Mus musculus, early embryonic cell, fibroblast. CIL. Dataset

NCBI Organism:Mus musculus; Cell Types:early embryonic cell, fibroblast; Cell Components:actin cytoskeleton, nucleus;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10016^* Cite

Description

NIH 3T3 cell (mouse embryonic fibroblast line) stained for Actin (green) and DNA (blue). PLoS Biology February 2009|Volume 7|Issue 2| e1000038 Active-Site Inhibitors of mTOR Target Rapamycin-Resistant Outputs of mTORC1 and mTORC2 Morris E. Feldman, Beth Apsel, Aino Uotila, Robbie Loewith, Zachary A. Knight, Davide Ruggero, Kevan M. Shokat

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: early embryonic cell; fibroblast
Cell Line: NIH/3T3
Cellular Component: actin cytoskeleton; nucleus

Attribution

Published: Feldman ME, Apsel B, Uotila A, Loewith R, Knight ZA, et al. (2009) Active-Site Inhibitors of mTOR Target Rapamycin-Resistant Outputs of mTORC1 and mTORC2. PLoS Biol 7(2): e1000038. doi:10.1371/journal.pbio.1000038
Link: http://www.plosbiology.org/article/info:doi/1...

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10016
Archival Resource Key (ARK): ark:/b7295/w9cil10016

Imaging

Image Type: wide-field detection
Image Mode: fluorescence microscopy; fluorescence emission; widefield illumination
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI); phalloidin; Alexa Fluor 488
Processing History: charge coupled device (CCD)

Sample Preparation

Methods: formaldehyde fixed tissue