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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10011*  Cite 
Description

Didinium has two bands of ciliated basal bodies that encircle the cell, one adorally and one aborally. The bands are composed of groups of approximately 15 ciliated basal bodies. This section is a longitudinal section of the cilium and basal body and cross section of approximately seven different groups or pectinelles. The proximal end of basal bodies are ensheathed in electron opaque sleeves from which microtubular ribbons extend into the adjacent ridges on the two sides of each basal body. TEM taken on 5/20/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X.Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:4664) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Didinium nasutum
Cell Type
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Carolina Biological Supply Company, NC, U.S.A.
Cellular Component
cell cortex
cilium
microtubule basal body
ciliary rootlet
axoneme
ectosymbiotic bacteria
Attribution
Names
Richard Allen
Link
http://www5.pbrc.hawaii.edu/allen/
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10011
Archival Resource Key (ARK)
ark:/b7295/w9cil10011
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 3400px ——
Y 2725px ——