TIP activity of Alexa 488–tagged EB1 protein (a plus end tracking protein). This movie shows pseudocolored overlaid images of rhodamine-labeled microtubules (red) and Alexa 488–tagged EB1 protein ...

Lattice diffusion of CLIP-170(H2)–GFP protein (a carboxyl truncation of the plus end microtubule tracking protein CLIP-170). The movie shows pseudocolored overlaid images of rhodamine-labeled microt...

Microtubules in a PtK1 cell injected with the Pak inhibitory fragment PBD/ID(H83L). Microtubules do not exhibit retrograde flow and undergo very little net growth. Epifluorescence image series were ac...

Microtubules in a control PtK1 cell at the edge of an epithelial cell island. Few pioneer microtubules rapidly grow into forming protrusions. The cell was microinjected with X-rhodamine-conjugated tub...

Microtubules in a constitutively active Rac1(Q61L)-expressing PtK1 cell. Microtubules are subjected to continuous retrograde flow away from the cell edge, but many plus ends undergo net growth and rem...

Microtubules in a dominant-negative Rac1(T17N)-expressing PtK1 cell. Microtubules do not undergo retrograde flow, and microtubules rapidly switch between growth and shortening phases with little net c...

Three dimensional data set acquired using a fast z-scan along the apical-to-basilar axis of a polarized epithelial cell in vitro to capture GFP-EB1 comets assosiated with the plus-end of actively poly...

The retrograde flow of microtubules oriented perpendicular to the leading edge inthe lamellipodium is not coupled to the movement of immediately adjacent lammellipodium. Primary cultures of newt (Tar...

Video shows the dynamics of microtubules (MTs) using a GFP fusion construct of EB3 (end binding protein 3) that transiently binds to growing MT plus ends, generating a punctate pattern of EB3-GFP come...

Video shows the dynamics of microtubules (MTs) using a GFP fusion construct of EB3 (end binding protein 3) that transiently binds to growing MT plus ends, generating a punctate pattern of EB3-GFP come...