Quick-freeze deep-etch of the contractile vacuole E-fracture face. This face like the collecting canal membrane is porous indicating that many IMPs have been removed from this leaflet during fracturin...

Quick-freeze deep-etch rotary-shadowed replica of a portion of the chemically-unfixed cytopharynx. The cytopharynx is continuous with the plasma membrane and is covered by only one membrane which is p...

The E-fracture faces of the plasma membrane and cytopharyngeal membranes are continuous but differ in appearance since the cytopharyngeal membrane is studded with IMPs while the plasma membrane has fe...

Details of the binding of the carrier vesicle to a microtubule. Four bridges, thought to be cytoplasmic dynein arms, span the 40nm space between these two structures. The membrane of the vesicle has b...

A freeze-fracture image of a DV-II transforming into a DV-III by rapid fusion of lysosomes with its membrane. The P-face shown here of the early DV-III has abundant IMPs as well as many inward blebs (...

Detail of fractured early Digestion Vacuole-III membrane showing the many IMPs on the P-fracture faces of the lysosomal membranes that had just fused with the DV. The membrane will smooth out as the p...

In quick-freeze deep-etch micrographs it is not easy to distinguish peroxisomes from surrounding organelles. The organelle at the top of this micrograph may be a peroxisome based on its finely granula...

A freeze-fractured view of the nuclear envelope. The fracture in the middle of the figure exposes the cytosolic facing membrane. This can be distinguished by a fractured cytoplasmic organelle at the t...

In quick-freeze deep-etch preparations the plasma membrane can be seen to contain plaques or plates of intramembrane particles (IMPs) that are regularly aligned in rows with a center-to-center spacing...

Freeze fracture image of the surface ridges at the anterior end of the cell where kineties to the right and left sides come together at an angle at the anterior suture tip. In this area are numerous p...