Saccharomyces cerevisiae (WT BY4741-MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) undergoing chronological aging on small synthetic 2% glucose media (SMD) with supplementation of 4 mM spermidine. Spermidine ...

Intracellular localization of GFP-Vps74 W88A, R97A mutant in BY4742 sac1∆ vps74∆ mutant. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-Vps7...

Intracellular localization of GFP-GOLPH3 in BY4742 vps74∆ sac1∆. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-GOLPH3 localizes, in additio...

Intracellular localization of GFP-GOLPH3 R171A, R174A mutant in BY4742 sac1∆ vps74∆ mutant. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-G...

Intracellular localization of GFP-Vps74 in a wild-type S. cerevisiae background grown at 37C. In wild-type cells incubated at 26C or 37C, GFP-Vps74 is localized to Golgi compartments and the cytosol. ...

Intracellular localization of GFP-Vps74 in S. cerevisiae frq1-1 temperature-conditional strain grown at permissive temperature of 26C (control for 37C temperature-shift). In frq1-1 mutant after a 30 m...

Intracellular localization of GFP-Vps74 in S. cerevisiae frq1-1 temperature-conditional strain grown at restrictive temperature of 37C. In frq1-1 mutant after a 30 minute incubation at the restrictive...

Intracellular localization of GFP-GOLPH3 in a wild-type S. cerevisiae background grown at 26C. In wild-type cells incubated at 26C or 37C, GFP-GOLPH localizes to intracellular membranes more robustly ...

Intracellular localization of GFP-GOLPH3 in S. cerevisiae frq1-1 temperature-conditional strain grown at restrictive temperature of 37C. In frq1-1 mutant after a 30 minute incubation at the restrictiv...

Intracellular localization of GFP-Vps74 K178A, R181A mutant in BY4742 vps74∆. GFP-Vps74 is localized to Golgi compartments and the cytosol (CIL# 13449) but loses localization when two amino acids ar...