Intracellular localization of GFP-Vps74 in S. cerevisiae pik1-83 temperature-conditional strain grown at permissive temperature of 26C (control for 37C temperature-shift). In pik1-83 mutant after a 30...

Intracellular localization of GFP-Vps74 in S. cerevisiae pik1-83 temperature-conditional strain grown at restrictive temperature of 37C. In pik1-83 mutant after a 30 minute incubation at the restricti...

Kre2-GFP is not efficiently maintained in the Golgi apparatus when Pik1-mediated PtdIns4P synthesis (the Golgi-localized PtdIns 4-kinase in yeast is composed of the Pik1 catalytic subunit and a myrist...

Intracellular localization of GFP-Vps74 in BY4742 sac1∆ vps74∆. SAC1 encodes an integral membrane phosphoinositide phosphatase that is localized to the ER and Golgi. In the sac1∆ vps74∆ mutant...

Nuclear envelope and spindle dynamics when the nucleus moves into the neck of kar9delta Nip100deltaCAP-Gly double mutant cells arrested in S phase. In these cells, the spindle failed to enter the bud ...

Spindle oscillation in wild-type cells arrested in S phase. In wild-type cells, the spindle moves in both directions through the bud neck; these movements coincide with lateral sliding of a cytoplasmi...

Spindle oscillation in kar9delta cells arrested in S phase. In wild-type cells, the spindle moves in both directions through the bud neck; these movements coincide with lateral sliding of a cytoplasmi...

Timelapse movie of GFP-labeled microtubules in kar9delta cells (arrested in S-phase) combined with a deletion of the CAP-Gly domain of Nip100. The number of cells in which the spindle moved from the m...