Actin dynamics in a Rac1(Q61L)-expressing PtK1 cell injected with Pak inhibitory fragment PBD/ID(H83L). Fast retrograde flow still occurs in the lamellipodium, but the width of the lamellipodium is re...

Microtubules in a constitutively active Rac1(Q61L)-expressing PtK1 cell. Microtubules are subjected to continuous retrograde flow away from the cell edge, but many plus ends undergo net growth and rem...

Microtubules in a dominant-negative Rac1(T17N)-expressing PtK1 cell. Microtubules do not undergo retrograde flow, and microtubules rapidly switch between growth and shortening phases with little net c...

Metaphase PtK1 cell microinjected with X-rhodamine-labeled tubulin and Alexa 488-labeled CENP-F antibodies to fluorescently label kinetochore fibers (red) and kinetochores/spindle poles (green), respe...

Mitotic PtK1 cell microinjected with X-rhodamine-labeled tubulin and Alexa 488-labeled CENP-F antibodies to fluorescently label kinetochore fibers (red) and kinetochores/spindle poles (green), respect...

Figs. 307 & 308 from Don Fawcett's Chapter 12 (Centrioles). In interphase cells, microtubules commonly radiate from the centrosome, and in dividing cells the microtubules of the mitotic spindle conver...

Mitotic PtK1 cell microinjected with X-rhodamine-labeled tubulin and Alexa 488-labeled CENP-F antibodies to fluorescently label kinetochore fibers (red) and kinetochores/spindle poles (green), respect...

Metaphase PtK1 cell microinjected with X-rhodamine-labeled tubulin and Alexa 488-labeled CENP-F antibodies, to fluorescently label kinetochore fibers (red) and kinetochores/spindle poles (green), resp...

The movie starts in metaphase and shows sister kinetochore pairs aligned at the metaphase plate and oscillating between the two spindle poles as their respective kinetochore fibers lengthen and shorte...

Details of the Golgi apparatus seen in 40 nm epon sections of Ptk tissue culture cells after plunge freezing,and freeze substitution. Image recorded at 28,500x with a Philips CM10 TEM operated at 80KV