3HA-Bfa1 (red) is now symmetrically localized to both spindle pole bodies (SPB) in cells with constitutive targeting of Tem1 to both SPBs in anaphase as determined by spindle morphology (tubulin, gree...

In tem1Δ cells expressing a Cnm67-Tem1 fusion protein, Cdc15-3HA (red) loads prematurely onto spindle pole bodies during metaphase as determined by spindle morphology (tubulin, green) and nuclear mor...

In tem1Δ cells expressing a Bfa1-Tem1 fusion protein, Cdc15-3HA (red) loads prematurely onto spindle pole bodies during metaphase as determined by spindle morphology (tubulin, green) and nuclear morp...

3HA-Cdc14 (red) localization in metaphase. The protein is sequestered in the nucleolus. Metaphase is determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Image is Fig...

Starved Dictyostelium cells lacking cGMP-binding protein C (gbpC-; no guanylyl cyclase activity) and in medium supplemented with 60 µM LY294002 (a PI3K inhibitor) and 1µM cAMP migrate toward the ano...

Cdc15-3HA (red) does not load onto spindle pole bodies in wild-type cells in metaphase as determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Control image for CIL# ...

3HA-Cdc14 (red) localization in anaphase. The protein is released throughout the whole cell. Anaphase is determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Image is...

3HA-Bfa1 (red) is generally localized to one spindle pole body (SPB) in wild-type cells in metaphase as determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Control i...

3HA-Bfa1 (red) is generally localized to the daughter spindle pole body (SPB) in wild-type cells in anaphase as determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). C...

Tem1 (green) normally localizes preferentially to the spindle pole body (SPB) that enters the daughter cell during anaphase; however, Tem1's ability to localize to the SPB is severely decreased in bfa...