In some cells exposed to horseradish peroxidase (HRP) for 30 sec and then immediately fixed some HRP has already entered the cell by endocytosis and has been removed from the early endosomes in carrie...

Acidosomes originate from the biosynthetic pathway that includes the ER, the Golgi stack and the trans-Golgi network (TGN). Flattened vesicles from the TGN round up into spherical vesicles and bind to...

Detail of acidosome docking sites at DV-IA planar view through the site where acidosomes are docked and/or fusing with a DV-I. These sites are labeled for the mAb specific for antigen L1. TEM taken on...

This micrograph shows that the discoidal vesicles meet the cytopharyngeal membrane edge-on where they fuse. Besides the ribbon of 12 microtubules abutting the membrane there is a second small ribbon o...

The cytosol of Paramecium contains many vesicles of various sizes, shapes and contents as well as complex particles such as ribosomes and glycogen granules. The rough endoplasmic reticulum exists as m...

Higher magnification of acidosomes docked at the nascent digestive vacuole membrane. The E-fracture face is relatively IMP free while the P-fracture face has many large IMPs as well as some sizable pi...

Quick-freeze deep-etch of acidosomes show the results of carrier vesicle fusion with the acidosomes. Both the E-face and P-face of the acidosomes appear to have two areas, the larger area on the E-fac...

The cytoproct is the cell’s defecation site where spent digestive vacuoles fuse with the plasma membrane. In this cell the cytoproct is closed and forms a ridge along the posterior suture on the ven...

Immunogold label specific for Q2 antigen on discoidal vesicles. The same mAb used in CIL:36721 heavily labels discoidal vesicles and less heavily the cytopharyngeal membrane. A few gold particles are ...

Quick-freeze deep-etch of acidosomes show the results of carrier vesicle fusion with the acidosomes. Both the E-face and P-face of the acidosomes appear to have two areas, the larger area on the E-fac...