Association of plectin with MTs. Electron microscopy of gelsolin-treated REF-52 cells after immunogold (10 nm) labeling for plectin. Plectin forms bridges between microtubules and intermediate filamen...

Association of plectin with MTs. Electron microscopy of gelsolin-treated REF-52 cells after immunogold (10 nm) labeling for Tyr-tubulin. Plectin forms bridges between microtubules (MTs) and intermedia...

A confocal 4D-stack from a transgenic Drosophila embryo during prometaphase expressing lamin-GFP (green) and injected with rhodamine-conjugated tubulin (red) and the lamin-B tail dominant negative sub...

A partially filled contractile vacuole is connected to two regions of spongiome tubules. The extra CV membrane is acquired from these spongiomes. Presumably some of these spongiome tubules are decorat...

A circular depression on the dorsal surface of the cell marks the expulsion site of a contractile vacuole. This is referred to as the contractile vacuole “pore.". Microtubules encircle the pore. Tr...

Contractile vacuole pore in face view illustrating the microtubular ribbons that extend from the pore to the CV membrane, the coil of microtubules that surrounds the pore and smaller pellicular pores ...

Detail of CV pore. Microtubules encircle the pore wall and other microtubules radiate out from the pore toward the CV membrane. TEM taken on 3/27/69 by R. Allen with Philips 300 operating at 60kV. Neg...

Detail of CV pore. Microtubules encircle the pore wall and other microtubules radiate out from the pore toward the CV membrane. TEM taken on 3/27/69 by R. Allen with Philips 300 operating at 60kV. Neg...

The contractile vacuole pore is coated on its cytosolic side by helically wound microtubules. Other microtubules abut this helix and extend out to the surface of the contractile vacuole where they are...

A high resolution of an oblique section of the Coleps contractile vacuole pore revealing the supporting microtubules and adjacent fibrous layer. Standard glutaraldehyde fixation followed by osmium tet...