Vorticella convallaria fixed for EM and buffered in cacodylate. The rough ER is not vesiculated as it is in collidine buffered fixatives but appears as sheets from which Golgi dictyosomes arise. A foo...

As with acidosome fusion with the DV-I, lysosome fusion with the DV-II also seems to occur nearly all at once resulting in a phagolysosome (DV-III) with a highly irregular surface. However, unlike the...

In order to study all stages of DVs in the same cell we used a protocol of sequentially exposing cells to different sizes of latex beads interspersed with washes: for example, 3 minutes in 0.1µm bead...

This is one of six images in Figure 6 in Apaja et al., JCB 2010 that shows the fate of various model membrane proteins, that are either folded or in the unfolded state at the plasma membrane, expresse...

This is one of six images in Figure 6 in Apaja et al., JCB 2010 that shows the fate of various model membrane proteins, that are either folded or in the unfolded state at the plasma membrane, expresse...

This is one of six images in Figure 6 in Apaja et al., JCB 2010 that shows the fate of various model membrane proteins, that are either folded or in the unfolded state at the plasma membrane, expresse...

The acidosome-like vesicle has a bull’s eye appearance with the invagination appearing in the middle of the vesicle. Other vesicles contain multiple layers resembling multilipid layers. Two vesicles...

Cytochalasin B blocks DV release from cytopharynx. In this micrograph a very large nascent vacuole has been retained at the cytopharynx of this cell that had been exposed to cytochalasin B. Many acido...

The image shows a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrifi...

This image shows the heterogeneity in the content of lysosomes (Lys) near the plasma membrane between two hepatocytes and near the bile canaliculus (not visible in this field). Some of the lysosomes ...