Intracellular localization of GFP-Vps74 K178A, R181A mutant in BY4742 sac1∆ vps74∆ mutant. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-Vp...

Intracellular localization of GFP-GOLPH3 W81A, R90A mutant in BY4742 sac1∆ vps74∆ mutant. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-GOL...

Kre2-GFP is efficiently maintained in the Golgi apparatus in BY4742 vps74∆ expressing wild-type, untagged, Vps74. Control image for images showing that localization of Kre2-GFP is lost when mutant f...

Kre2-GFP is not efficiently maintained in the Golgi apparatus when Pik1-mediated PtdIns4P synthesis ceases, implicating Pik1 signaling in retrograde trafficking of Golgi residents. In this image, Kre2...

Wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) colocalizes primarily at the Golgi (Giantin) (red) with Helix Pomatia Lectin (HPL) (green) in unstimulated HeLa cells. HPL binds various glycans but the Tn ant...

Constitutively active mutant Arf1-GFP (Arf1(Q71L)-GFP) (gray) colocalizes primarily at the Golgi (Giantin) (red) with Helix Pomatia Lectin (HPL) (green) in unstimulated HeLa cells. HPL binds various g...

Helix Pomatia Lectin (HPL) (green) is redistributed out of the Golgi (Giantin) (red) in HeLa cells upon EGF stimulation for 4h. The Tn antigen refers to terminal α-linked N-acetyl galactosamine resid...

An ER-trapped GalNac-T activity reporter (Muc-PTS; fused to GFP) (green) is targeted to the ER marked by PDI (protein disulfide isomerase) (red) in HeLa cells cotransfected with inactive Src (SrcK-mCh...

An ER-trapped GalNac-T activity reporter (Muc-PTS; fused to GFP) (green) is targeted to the ER and not the Golgi, marked by Giantin (red) in HeLa cells cotransfected with inactive Src (SrcK-mCherry, c...

Constituvely active mutant Arf1-GFP (Arf1(Q71L)-GFP) (gray) blocks redistribution of Helix Pomatia Lectin (HPL) (green) from Golgi to ER in Src-deficient SYF fibroblasts cotransfected with active Src...