The tubules arising from late DV-III expand at their distal ends into rounded shapes. These expanded ends contain a glycocalyx like the secondary lysosomes and large IMPs also like secondary lysosomes...

Only the macronucleus contains nucleoli which is the site of ribosomal RNA synthesis and ribosome subunit assembly. This EM also contains the distal end of the food vacuole forming cytopharynx which c...

Accumulations of acidosome-like vesicles are sometimes found (in this case in a predividing cell) and in this micrograph provide further evidence for invaginations of the outer membranes. It appears t...

In some cells exposed to horseradish peroxidase (HRP) for 30 sec and then immediately fixed some HRP has already entered the cell by endocytosis and has been removed from the early endosomes in carrie...

Acid phosphatase is present in tubules retrieved from late DV-III. The tubules and rounded expansions arising as a result of late DV-III membrane retrieval contain an electron-opaque reaction product ...

High resolution micrograph following trifluoperizine (TFP) treatment, which apparently does not inhibit endocytosis but does inhibit movement of vesicles away from the parasomal sacs, coated and preen...

Quick-freeze deep-etch images show a more three dimensional view of acidosomes surrounding a phagosome. Acidosomes are unique in that they contain indentations of their membrane. The lumen has a honey...

A high magnification view of the intimate docking of acidosomes with the phagosome is particularly apparent where the P-face of the acidosome lies against the E-face of the phagosome. Numerous large I...

The cytopharynx/nascent digestive vacuole (phagosome) has an E-fracture face containing a high number of IMPs. Interestingly these IMPs are sometimes organized into plaques where there are several sho...

High resolution image of two parasomal sacs that are oriented perpendicular to the basal bodies. The top parasomal sac has a clathrin cage around it's coated pit area. The P-face of the plasma membran...