Localization of Alexa Fluor-594-α-factor-labeled endosomes (center; red in merge) and Abp140-3GFP (left; green in merge). Abp140p binds F-actin and localizes to actin patches and cables. Endosome mov...

Detail of acidosome docking sites at DV-IA planar view through the site where acidosomes are docked and/or fusing with a DV-I. These sites are labeled for the mAb specific for antigen L1. TEM taken on...

Internalization of Alexa 594-a-factor. When added to cells, A594-α-factor (and A488-α-factor, data not shown) was first seen in internal endocytic compartments by 5 min. By 10 min, A594-α-factor be...

Localization of Abp1-RFP (red; left) and Abp140-3GFP (green; center). By expressing Abp1-mRFP and Abp140–3GFP to visualize endocytic vesicles and actin cables respectively, we observed that approxim...

Time-lapse confocal experiment showing deltorphin II (100 nM; a selective agonist)-induced receptor internalization in primary caudate putamen neurons isolated from Oprd1-EGFP/EGFP knockin mouse. All ...

Phagosome neutralization visualized using FITC-yeast. Amoebae of Dictyostelium discoideum were fed living budding yeast (S. cerevisiae strain TH2-1B) that had been labeled with fluorescein isothiocyan...

A movie of phagosome neutralization visualized using FITC-yeast. Amoebae of Dictyostelium discoideum were fed living budding yeast (S. cerevisiae strain TH2-1B) that had been labeled with fluorescein ...

Localization on the cell surface of Alexa Fluor-488-α factor (left, green in merge) and Ede1-RFP (center; red in merge). The Ede1p spots in the white boxes appear first and are then joined by α fact...

As with acidosome fusion with the DV-I, lysosome fusion with the DV-II also seems to occur nearly all at once resulting in a phagolysosome (DV-III) with a highly irregular surface. However, unlike the...

A movie that shows phagosome acidification and neutralization visualized using FITC-yeast. Amoebae of Dictyostelium discoideum were fed living budding yeast (S. cerevisiae strain TH2-1B) that had been...