Time series of cooled CCD fluorescence micrographs of live amoeba syringe-loaded with Rho-N58C-PII. See publication below for details. Live cells were observed with a Zeiss Axiovert 135 fluorescence m...

The carrier vesicles dock with the acidosomes and then fuse with these larger vesicles before or as the vesicles dock at the nascent Digestive Vacuole. Thus the contents of the carrier vesicles are fi...

The carrier vesicles dock with the acidosomes and then fuse with these larger vesicles before or as the vesicles dock at the nascent Digestive Vacuole. Thus the contents of the carrier vesicles are fi...

Time-lapse experiments showing deltorphin II (100 nM; a selective agonist)-induced internalization of delta-opioid receptor–enhanced GFP (DOR-EGFP; green) receptors expressed in HEK 293 cells. The C...

A DV-III contains a thick continuous glycocalyx (polysaccharide coat) on the luminal side of its membrane as well as paracrystalline material, both provided by the fused lysosomes. The latex beads ind...

The nascent digestive vacuole forms as the membrane of flattened vesicles fuse with the single membrane of the cytopharynx between the lamellae (2 microtubules forming a lamella) that are connected al...

Localization of Alexa Fluor-594-α-factor-labeled endosomes (red), Abp1-GFP- (green) and Abp140-3GFP- (green) labeled endocytic vesicles. It was possible to distinguish endocytic vesicles from actin c...

Dynamics of membrane ruffling (top) and intracellular vesicle motion (bottom) in a cSrc-transfected COS-7 cell. Images were collected with a Bessel Beam Microscope using two photon excitation. This f...

In more extreme cases microfilamentous material builds up into a thick layer surrounding the DV-I membrane as well as the fusion sites between the acidosomes and the DV-I. A similar thick meshwork was...

Localization of Alexa Fluor-594-α factor labeled endosomes (red) and Sla1-GFP labeled endocytic vesicles (green). Note that several vesicles are targeted to the prominent endosome in the (upper) moth...