Human colon carcinoma HCT 116 cells were transfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and enucleated to obtain cytoplasts. The cytoplasts were treated with vehicle...

Image indicative of 24 h autophagic activity of human colon carcinoma HCT 116 cell co-transfected with a RFP-LC3-encoding plasmid and a SIRT1 fused to GFP plasmid. Confocal fluorescent images were cap...

Human colon carcinoma HCT 116 cells were transfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and treated with vehicle for 4 h as a control for EX527 and resveratrol or sp...

Human colon carcinoma HCT 116 cells were transfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and treated with 100µM Spermidine for 4 h. For fluorescence microscopy deter...

Human colon carcinoma HCT 116 cells were transfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and treated with 100 µM Spermidine in the presence of SIRT1 inhibitor EX527 ...

Traffic Jam: The Dynamic Behavior of a Golgi Matrix Protein This video shows an NRK cell stably expressing the Golgi matrix protein GRASP55-GFP. The Golgi apparatus in mammalian cells is made up of ...

Human colon carcinoma HCT 116 cells were transfected with an siRNA specific for SIRT1 and then retransfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and left untreated fo...

Human colon carcinoma HCT 116 cells were transfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and enucleated to obtain cytoplasts. The cytoplasts were treated with 100 µM...

Human colon carcinoma HCT 116 cells were transfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and enucleated to obtain cytoplasts. The cytoplasts were treated with 100 µM...

Image indicative of 24 h autophagic activity of human colon carcinoma HCT 116 cell co-transfected with a RFP-LC3-encoding plasmid and a SIRT1 variant with a mutation in the nuclear localization signal...