Acid phosphatase is synthesized in the biosynthetic pathway in the ER and packaged in the TGN into primary lysosomes which are passed on to secondary lysosomes. Primary lysosomes are small vesicles of...

PrA contains a distinct determinant for glycan-independent ERAD (ER associated degradation). A mutant of the vacuolar proteinase A (PrA*-Gldelta4) (green) is a substrate of ER quality control (ERQC) m...

Protein translocation is the process by which peptides are transported across a membrane bilayer. Translocation of proteins across the membrane of the endoplasmic reticulum (ER) is know to occur in on...

Protein translocation is the process by which peptides are transported across a membrane bilayer. Translocation of proteins across the membrane of the endoplasmic reticulum (ER) is know to occur in on...

Mitochondria in HeLa cells are identified by mito-YFP (green) following treatment with the mitochondrial depolarizing agent CCCP (carbonyl cyanide m-chlorophenyl hydrazone). When fixed cells are perme...

PINK1-YFP R98F (green) is localized to mitochondria in the absence of the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone). The R98F mutation in PINK1-YFP partially in...

YFP-WT PINK1 (green) shows mitochondrial localization in HeLa cells following treatment with the mitochondrial depolarizing agent CCCP (carbonyl cyanide m-chlorophenyl hydrazone). When fixed cells are...

Mitochondria in HeLa cells are identified by mito-YFP (green). When fixed cells are unpermeabilized (not treated with detergent), antibodies to cyctochrome c (red), a mitochondrial matrix marker, and ...

Mitochondria in HeLa cells are identified by mito-YFP (green). When fixed cells are permeabilized with 0.25% Triton X-100 (TX-100), both antibodies to cyctochrome c (red), a mitochondrial matrix marke...

Intracellular localization of GFP-GOLPH3 in a wild-type S. cerevisiae background grown at 37C. In wild-type cells incubated at 26C or 37C, GFP-GOLPH localizes to intracellular membranes more robustly ...