A population of CHO cells constitutively overexpressing a clone of human IgG2/kappa. CHO cells are regarded as the workhorse of recombinant protein expression and biopharmaceutical production. Under c...

COS 7 cell expressing Organelle LightsTM ER-GFP (Invitrogen) consisting of the signal sequence of the ER protein calreticulin and a KDEL retention sequence. Images were collected with a 63X 1.4 NA Pl...

Fibers that splay out from a mid piece pass to the pellicle. TEM taken on 4/5/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 10,250X. Bar = 1µm. Published and adapted with permission from ...

Fibers that splay out from a mid piece pass to the pellicle. TEM taken on 4/5/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 10,250X. Published and adapted with permission from J. Cell Biol...

Large patches of rough ER surrounded by Golgi stacks (visible only at high magnification) have formed in the cytosol along with fields of lipid bodies. By day 2 these ER and lipid fields will be dispe...

The endoplasmic reticulum exists in extensive sheets in the cytoplasm of Nassula immediately below the alveolar membranes. TEM taken on 3/14/69 by R. Allen with Philips 300 operating at 60kV. Neg. 16,...

V. convallaria preserved in collidine buffered fixative (not cacodylate) has a less dense cytoplasm in which oranelles are easier to recognize. The rough ER has vesiculated but this vesiculation enhan...

V. convallaria preserved in collidine buffered fixative (not cacodylate) has a less dense cytoplasm in which oranelles are easier to recognize. The rough ER has vesiculated but this vesiculation enhan...

The linkage complex consists of a spindle shaped mid piece bordered, in one plane, by several rails that have subunits that pass through the ER membrane. Thin filaments splay out from both tips of the...

A cross-section and tangential views of three other linkage complexes. The mid piece sits in an indentation of the ER membrane on a bed of 5 slats that pass through the membrane. Rails affix the ER me...