Quick-freeze deep-etch image of a DV-III containing paracrystalline sheets in its lumen. The sheets have parallel rows of subunits spaced at 20nm center-to-center but the chemical nature of the sheets...

To see if acidosomes contain hydrolases (digestive enzymes) we incubated cells in Gomori’s medium which in the presence of acid phosphatase and lead will form an electron-opaque reaction product. Ac...

Secondary lysosomes in quick-freeze deep-etch images lie against the smooth E-face of a phagoacidosome (DV-II) membrane. The P-fracture face of one fairly large lysosome lies close to the DV-II but no...

Secondary lysosomes contain a fairly dense matrix in well fixed cells. The content of these lysosomes seems to be released as a package into the phagoacidosome when they fuse to form the phagolysosome...

The carrier vesicles dock with the acidosomes and then fuse with these larger vesicles before or as the vesicles dock at the nascent Digestive Vacuole. Thus the contents of the carrier vesicles are fi...

The deep fiber is associated with the cytopharyngeal membrane (see Smith and Bunse, Trans. Am. Microsc. Soc. 102:264-271, 1983). This membrane has acidosome-like vesicles docked at its cytosolic surfa...

The carrier vesicles dock with the acidosomes and then fuse with these larger vesicles before or as the vesicles dock at the nascent Digestive Vacuole. Thus the contents of the carrier vesicles are fi...

A view near the oral apparatus of Coleps and the attachment site of a microtubule filament bundle, fibrous interconnections, and a rod near a basal body that is out of the section plane in this TEM. D...

Tetrahymena cells that are not forming a digestive vacuole often have a tube-like extension of the cytopharyngeal membrane attached to the deep fiber that extends several microns into the cytoplasm. T...

The entire membrane of the phagolysosome does appear to have been changed into that similar to the lysosome membrane which bears a thick glycocalyx (polysaccharide coat) and no longer appears to be li...